High frequency somatic embryogenesis and plantlet regeneration from shoot tip explants of Soy bean (Glycine max .L.) Merr.)

Establishment of efficient protocol for the induction of somatic embryogenesis and plantlet regeneration from shoot tip explants was achieved in Glycine max (L).Merr. cv.PK472. Highest frequency (95%) of embryogenic callus formation was obtained from shoot tip explants of Glycine max (L).Merr.cv.PK472 on Murashige and Skoog (MS) medium fortified with different concentration of 2,4-Dichlorophenoxy acetic acid (2,4-D) and Benzyl amino purine (BAP) .Fast growing, yellowish nodular callus lines containing somatic embryos were established on initiation medium supplemented with 5.0mg/L 2.4-D and 1.0mg/L BAP respectively. Embryo development and maturation was achieved on MS medium supplemented with 5.0mg/L 2,4-D and 4.0mg/L BAP .The well formed embryos germinated into complete plantlet on MS medium containing 4.0mg/L BAP and 1.0mg/L NAA(Napthalene acetic acid).The regenerated plants ware first transferred to plastic cups and later into pots for ANNQUEST 2014 :3(1): 1-8 2 maturation. Regenerated plants did not exhibit observable morphological alternation.