Simultaneous Determination Of Hyoscine Butyl Bromide and Dipyrone in Their Binary Mixture By RP-HPLC Method
نویسندگان
چکیده
RP-HPLC chromatographic method was developed for determination of Hyoscine Butyl Bromide (HBB) and Dipyrone ( DIP). In this method, HBB and DIP were separated by using C18 (25 cm×4.6 mm i.d. 5μm particle size) column as a stationary phase and using water: methanol (50:50) v/v pH adjusted to 7.0 with triflouroacetic acid and triethylamine) as a mobile phase, maintaining the flow rate at 1.0 mL min with UV detection at 210 nm. The proposed method was successfully applied for determination of HBB and DIP in pure form and in their pharmaceutical formulations.
منابع مشابه
Simultaneous determination of hyoscine N-butyl bromide and paracetamol in their binary mixture by RP-HPLC method
RP-HPLC; Chromatography; Hyoscine N-butyl bromide and paracetamol Abstract RP-HPLC chromatographic method was developed for the determination of hyoscine N-butyl bromide (HBB) and Paracetamol (PAR). In this chromatographic method, HBB and PAR were separated using C18 (25 cm · 4.6 mm i.d. 5 lm particle size) column as a stationary phase and water: methanol (50:50, V/V pH adjusted to 3.9 with CF3...
متن کاملExtended-Gate Field-Effect Transistor based Sensor for Detection of Hyoscine N-Butyl Bromide in its Pharmaceutical Formulation
A novel recognition method for selective determination of the hyoscine N-Butyl bromide (HBB), an antispasmodic agent for smooth muscles, was devised using extended gate field-effect transistor (EG-FET) as transducing unit. For this purpose a PVC membrane, containing hyoscine n-butyl-tetraphenyl borate ion-pair as recognition component, was coated on Ag/AgCl wire, which was connected to the exte...
متن کاملSimultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form
The present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. The drug was dissolved in NaOH solution (0.1N NaOH) for estimation in UV and in distilled water fo...
متن کاملA Rapid, Stability-Indicating RP-HPLC Method for the Simultaneous Determination of Formoterol Fumarate, Tiotropium Bromide, and Ciclesonide in a Pulmonary Drug Product
A stability-indicating reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for the simultaneous determination of Formoterol fumarate (FOR), Tiotropium bromide (TRI), and Ciclesonide (CLS) in a pulmonary drug product. The desired chromatographic separation was achieved on the Zorbax SB C8, 5 μm (150 × 4.6 mm) column, using gradient elution at 230 nm detector wave...
متن کاملSimultaneous Determination of Sulfamethoxazole and Phthalazine by HPLC and Multivariate Calibration Methods
Two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of Sulfamethoxazole (SMX) and Phthalazine (PHZ) by High Performance Liquid Chromatography (HPLC). Multivariate calibration techniques such as Classical Least Squares (CLS) and Inverse Least Squares (ILS) were introduced into HPLC to determine the quantification by using UV d...
متن کامل