An improved assay of UV-induced thymine-containing dimers in Saccharomyces cerevisiae.
نویسندگان
چکیده
The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5'-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.
منابع مشابه
UV-induction of thymine-containing dimers in Saccharomyces cerevisiae.
In haploid and diploid S. cerevisiae the dimer yield ratio TT/CT is found to be 1.2/1 and 1.3/1, resp., at the UV (254 nm) unit dose 1 erg/mm2, the share of TT and CT in a UV (254 nm) lethal hit being 0.7 TT and 0.6 CT. A general formulation of the UV lethal hit is given and discussed. The TT+CT yields obtained for S. cerevisiae are compared to those reported for other organisms. It is found th...
متن کاملFate of thymine-containing dimers in the deoxyribonucleic acid of ultravioletirradiated Bacillus subtilis.
The fate of ultraviolet-induced, thymine-containing dimers in the deoxyribonucleic acid (DNA) of Bacillus subtilis was investigated in both the wild type (UV(R)) and an ultraviolet light-sensitive (UV(S)) mutant. During incubation in the dark, dimers were excised from the DNA of the UV(R)B. subtilis, but remained in the DNA of the UV(S) mutant. About 40% of the excised dimers recovered in the w...
متن کاملDistribution of ultraviolet-induced lesions in simian virus 40 DNA.
In order to analyze the molecular mechanisms of mutagenesis in mammalian cells, we devised an analytical assay using Simian Virus 40 as biological probe. To study the possible correlations between the distribution of the lesions on the treated DNA and the distribution of mutations, we have located and quantified the lesions induced by ultraviolet light (254 nm) on a SV40 DNA fragment. At a flue...
متن کاملSpore photoproduct (SP) lyase from Bacillus subtilis specifically binds to and cleaves SP (5-thyminyl-5,6-dihydrothymine) but not cyclobutane pyrimidine dimers in UV-irradiated DNA.
The predominant photolesion in the DNA of UV-irradiated dormant bacterial spores is the thymine dimer 5-thyminyl-5,6-dihydrothymine, commonly referred to as spore photoproduct (SP). A major determinant of SP repair during spore germination is its direct reversal by the enzyme SP lyase, encoded by the splB gene in Bacillus subtilis. SplB protein containing an N-terminal tag of six histidine resi...
متن کاملDifferential repair of UV damage in Saccharomyces cerevisiae is cell cycle dependent.
Preferential repair of UV-induced damage is a phenomenon by which mammalian cells might enhance their survival. This paper presents the first evidence that preferential repair occurs in the lower eukaryote Saccharomyces cerevisiae. Moreover an unique approach is reported to compare identical sequences present on the same chromosome and only differing in expression. We determined the removal of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Section C, Biosciences
دوره 30 6 شماره
صفحات -
تاریخ انتشار 1975