A data comparison between a traditional and the single-step β-galactosidase assay
نویسندگان
چکیده
This article describes reproducibility of a single-step automated β-galactosidase, and the equivalence of its data to the traditional assay ("Experiments in Molecular Genetics" [1]). This was done via a pairwise comparison of both methods using strains with Miller Unit [MU] values ranging from 0 to over 2000. The data presented in this article is associated with the research article entitled "A single-step method for mid to high throughput β-galactosidase assays in Escherichia coli using a microplate reader" [2].
منابع مشابه
One-step high-throughput assay for quantitative detection of beta-galactosidase activity in intact gram-negative bacteria, yeast, and mammalian cells.
(shaded bars; left scale) are presented beside β-gal activity units determined on the indicated strains in the same conditions by ONPG-based assays (empty bars; right scale), performed using the traditional Miller method for bacteria (3) and crude protein extracts for yeast (1). MUG, 4-methylumbelliferyl β-d-galactopyranoside; ONPG, o-nitrophenyl-β-d-galactopyranoside; β-gal; β-galactosidase. B...
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