Characterization of natural HIV-1 Tat and Vpr variants from Northern India
نویسندگان
چکیده
Methods HIV-1 Tat exon 1 and Vpr were amplified from the DNA isolated from blood of HIV infected patients and cloned. Clones were got sequenced and aligned against reference sequences using CLUSTAL W. Sim plot analysis was done for recombinants. Their expression was accessed by transfection of HEK 293T cells with myc fusion clones of variants and western blotting using anti-myc antibody. The variant Tat clones were cotransfected with LTR-luc to investigate their LTR transactivation potential by dual luciferase reporter assay.
منابع مشابه
Effect on HIV-1 Gene Expression, Tat-Vpr Interaction and Cell Apoptosis by Natural Variants of HIV-1 Tat Exon 1 and Vpr from Northern India
BACKGROUND Since HIV-1 Tat and Vpr genes are involved in promoter transactivation, apoptosis, etc, we carried out studies to find out nature and extent of natural variation in the two genes from seropositive patients from Northern India and determined their functional implications. METHODS HIV-1 tat exon 1 and vpr were amplified from the genomic DNA isolated from the blood of HIV-1 infected i...
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