The Electrical Activity of Embryonic Chick Heart Cells Isolated in Tissue Culture Singly or in Interconnected Cell Sheets
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چکیده
Wi th the advent of techniques for dissociating hear t tissue into its componen t cells and growing these cells in cul ture (for review see D e H a a n , 1967 a, b), a growing l i terature has developed on the electrophysiological properties of embryonic hear t cells ma in ta ined in vitro (e.g. F~nge et al., 1956; CriU et al., 1959; Sperelakis and Lehmkuh l , 1966; L e h m k u h l and Sperelakis, 1967).
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The Electrical Activity of Embryonic Chick Heart Cells Isolated in Tissue Culture Singly or in Interconnected Cell Sheets
Embryonic chick heart cells were cultured on a plastic surface in sparse sheets of 2-50 cells mutually in contact, or isolated as single cells. Conditions are described which permitted conjoint cells to be impaled with recording microelectrodes with 75% success, and isolated single cells with 8% success. It is proposed that cells in electrical contact with neighbors are protected from irreversi...
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