Interlaboratory comparison of serum vitamin B12 assay.
نویسندگان
چکیده
The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods, whereas excellent correlation, reproducibility, and recovery were obtained in reference laboratories by microbiological assay with both E. gracilis and L. leichmannii. In general, RID gave higher results than microbiological assay. The need for suitable reference sera is emphasised.
منابع مشابه
Vitamin B12 in human blood and serum. I. Comparison of microbiologic assays using normal subjects.
HE MICROORGANISMS used for Vitamin B12 (cyanocobalamin) assay are LaCtobaciUus lewhmanniz (1), Euglena gracilis (2), Ochromonas maihamensis (3-5), and a mutant strain of Escherichia COli (E. coli 113-3) (6). Improvements in protozoan assays for B12 show such assays to be more sensitive and specific than bacterial assays (2, 7, 8). Ochromonas maihamensis is highly specific for true forms of B12 ...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 33 3 شماره
صفحات -
تاریخ انتشار 1980