Cis-Regulatory Changes Associated with a Recent Mating System Shift and Floral Adaptation in Capsella
نویسندگان
چکیده
The selfing syndrome constitutes a suite of floral and reproductive trait changes that have evolved repeatedly across many evolutionary lineages in response to the shift to selfing. Convergent evolution of the selfing syndrome suggests that these changes are adaptive, yet our understanding of the detailed molecular genetic basis of the selfing syndrome remains limited. Here, we investigate the role of cis-regulatory changes during the recent evolution of the selfing syndrome in Capsella rubella, which split from the outcrosser Capsella grandiflora less than 200 ka. We assess allele-specific expression (ASE) in leaves and flower buds at a total of 18,452 genes in three interspecific F1 C. grandiflora x C. rubella hybrids. Using a hierarchical Bayesian approach that accounts for technical variation using genomic reads, we find evidence for extensive cis-regulatory changes. On average, 44% of the assayed genes show evidence of ASE; however, only 6% show strong allelic expression biases. Flower buds, but not leaves, show an enrichment of cis-regulatory changes in genomic regions responsible for floral and reproductive trait divergence between C. rubella and C. grandiflora. We further detected an excess of heterozygous transposable element (TE) insertions near genes with ASE, and TE insertions targeted by uniquely mapping 24-nt small RNAs were associated with reduced expression of nearby genes. Our results suggest that cis-regulatory changes have been important during the recent adaptive floral evolution in Capsella and that differences in TE dynamics between selfing and outcrossing species could be important for rapid regulatory divergence in association with mating system shifts.
منابع مشابه
1 2 3 Cis - regulatory changes associated with a recent mating system shift and floral 4 adaptation in Capsella
متن کامل
Analysis of allele-specific expression reveals cis-regulatory changes associated with a recent mating system shift and floral adaptation in Capsella
Kim A. Steige, Johan Reimegård, Daniel Koenig, Douglas G. Scofield, Tanja Slotte* Department of Ecology and Genetics, Uppsala University, Uppsala, Sweden Science for Life Laboratory, Uppsala University, Uppsala, Sweden Max Planck Institute for Developmental Biology, Tübingen, Germany Department of Ecology, Environment and Plant Sciences, Science for Life Laboratory, Stockholm University, Stockh...
متن کاملRecent mating-system evolution in Eichhornia is accompanied by cis-regulatory divergence.
The evolution of predominant self-fertilization from cross-fertilization in plants is accompanied by diverse changes to morphology, ecology and genetics, some of which likely result from regulatory changes in gene expression. We examined changes in gene expression during early stages in the transition to selfing in populations of animal-pollinated Eichhornia paniculata with contrasting mating p...
متن کاملRepeated Inactivation of the First Committed Enzyme Underlies the Loss of Benzaldehyde Emission after the Selfing Transition in Capsella
The enormous species richness of flowering plants is at least partly due to floral diversification driven by interactions between plants and their animal pollinators [1, 2]. Specific pollinator attraction relies on visual and olfactory floral cues [3-5]; floral scent can not only attract pollinators but also attract or repel herbivorous insects [6-8]. However, despite its central role for plant...
متن کاملThe Genetic Basis of Pollinator Adaptation in a Sexually Deceptive Orchid
In plants, pollinator adaptation is considered to be a major driving force for floral diversification and speciation. However, the genetic basis of pollinator adaptation is poorly understood. The orchid genus Ophrys mimics its pollinators' mating signals and is pollinated by male insects during mating attempts. In many species of this genus, chemical mimicry of the pollinators' pheromones, espe...
متن کامل