Influence of Immunostimmulant on Abdominal Muscle Aspartate Transaminase and Alanine Transaminase in Mice against Hepatitis B

Eight groups of male Swiss albino mice mice (6 8 weeks old; 23 26g wt) were employed to study the effect of Immunostimulant, (Immunex DS) on the level of abdominal muscle transaminases against Gen Vac B vaccine. In group I, Immunex DS (IDS) was orally administered (@ 150mg/mouse) to 10 mice, in 6 groups of mice (10 in each) IDS was orally administered @ 150mg/mouse on 0 day and Gen Vac B Vaccine @ 0.07 ml/mouse (group A), 0.1ml/mouse (group B), 0.2ml/mouse (group C), 0.4ml/mouse (group D), 0.8ml/mouse (group E) and 1ml/mouse (group F) was injected on day 4 of experiment. Another group (U) of mice (ten) was kept as controls for comparison (untreated with IDS + uninfected). Two mice from each experimental (A, B, C, D, E and F) and control groups U and I (after day 7 of vaccine treatment in case of experimentals) were necropsied, from day 1-5. Abdominal muscle tissue was separated and analyzed for transaminases using standard methods. Both aspartate transaminase (AST) and alanine transaminase (ALT) showed a considerable increase in all the experimental groups of mice when compared with controls (except the level of AST from day 3-5 in group F and ALT on day 5 in group A and on day 3-5 in group B) and IDS treated mice, (except the level of AST on day 1 and 2 in all the 6 groups and ALT from day 1-5 in groups A and B and on day 1 and 2 in groups C and D) during the day 1 30 of experimental period. The level of AST and ALT almost remained constant from day 1 to 5 of experiment in IDS treated mice. Though liver is the major organ to be influenced during toxic reactions and/or microbial infection, the process of tranasamination of enzymes is found to be disturbed in all the experimental groups of mice which received various doses of vaccine after immunostimulation. It is evident that IDS and/or vaccine might have caused stress resulting in the marked alteration in the level of transaminases in the abdominal muscles of mice.