Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor

Several kinds of modified carbon screen printed electrodes (CSPEs) for amperometric detection of hydrogen peroxide (H₂O₂) are presented in order to propose a methyl mercaptan (MM) biosensor. Unmodified, carbon nanotubes (CNTs), cobalt phthalocyanine (CoPC), Prussian blue (PB), and Os-wired HRP modified CSPE sensors were fabricated and tested to detect H₂O₂, applying a potential of +0.6 V, +0.6 V, +0.4 V, -0.2 V and -0.1 V (versus Ag/AgCl), respectively. The limits of detection of these electrodes for H₂O₂ were 3.1 μM, 1.3 μM, 71 nM, 1.3 μM, 13.7 nM, respectively. The results demonstrated that the Os-wired HRP modified CSPEs gives the lowest limit of detection (LOD) for H₂O₂ at a working potential as low as -0.1 V. Os-wired HRP is the optimum choice for establishment of a MM biosensor and gives a detection limit of 0.5 μM.