DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.
نویسندگان
چکیده
DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 41 4 شماره
صفحات -
تاریخ انتشار 2003