The Neonatal Development of Intraepithelial and Lamina Propria Lymphocytes in the Murine Small Intestine

Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine.

Existing methods for the production of lymphocytes from the small intestine have proved unsatisfactory when applied to the mouse. We report here a new method for the production of highly pure suspensions of lymphoid cells from the epithelial layer and lamina propria of mouse small intestine. The production and purification methods are described in detail. At least ten million lymphocytes are ob...

Demonstration of functional role of TECK/CCL25 in T lymphocyte-endothelium interaction in inflamed and uninflamed intestinal mucosa.

It has recently been suggested that C-C chemokines may play a role in the organ-specific homing of lymphocytes, but there is not enough in vivo evidence in intestinal mucosa. The aim of this study was to examine whether thymus-expressed chemokine (TECK)/CCL25 and its ligand CCR9 are involved in T-lymphocyte interaction with microvessels of murine intestinal mucosa. T lymphocytes from the small ...

Trichuris muris Nematode Parasite Intestine of Mice Infected with the Intestinal and Isolated Lymphoid Follicles in the Large Lymphocytes, Lamina Propria Leukocytes, The Characterization of Intraepithelial

Improved isolation methods for mucosal leukocytes from small and large intestines in rats.

We optimized the isolation protocol for intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) from the rat small intestine, and LPLs from even the rat large intestine. The major population of IELs in the small intestine was considered to be from the villus epithelia. The cytotoxicity of mucosal leukocytes was comparable among isolated fractions from both the small and large i...

Intestinal Lamina Propria TcRγδ+ Lymphocytes Selectively Express IL-10 and IL-17

Background: The characteristics and roles of gut lymphocytes have been only partly elucidated, in particular with regard to activation patterns. Objectives: To characterize lymphocytes from various parts of the gut and examine their activation pattern as a network. Methods: Lymphocytes were isolated from the epithelium, the lamina propria, Peyer's patches, mesenteric lymph nodes, the spleen, an...