Detection of Countable Number of Molecules by Enzyme-linked Immunosorbent Assay in Extended Nanochannels
نویسندگان
چکیده
We developed a nanofluidics-based enzyme-linked immunosorbent assay (ELISA) device and realized quantification of countable number of molecules by combining enzymatic amplification reaction and ultrasensitive detection with a differential interference contrast thermal lens microscope (DIC-TLM). Our method will contribute to single cell analysis field, in particular for ultrasensitive protein counting.
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