Ribonomic and Mechanistic Analysis of the Human Pum 1 RNA Binding Protein
نویسندگان
چکیده
Ribonomic and Mechanistic Analysis of the Human Pum1 RNA Binding Protein by Adam Remy Morris University Program in Genetics and Genomics Duke University Date:_______________________ Approved: ___________________________ Jack Keene, Chair ___________________________ David Pickup ___________________________ Jen-Tsan Chi ___________________________ Uwe Ohler ___________________________ Dennis Thiele An abstract of a dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the University Program in Genetics and Genomics in the Graduate School of Duke University 2010 Copyright by Adam Remy Morris 2010
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Identification of diverse target RNAs that are functionally regulated by human Pumilio proteins
Human Pumilio proteins, PUM1 and PUM2, are sequence specific RNA-binding proteins that regulate protein expression. We used RNA-seq, rigorous statistical testing and an experimentally derived fold change cut-off to identify nearly 1000 target RNAs-including mRNAs and non-coding RNAs-that are functionally regulated by PUMs. Bioinformatic analysis defined a PUM Response Element (PRE) that was sig...
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