The Effect of Bioactive Glass Nanoparticles on the Behavior of Human Periodontal Ligament Cells
نویسندگان
چکیده
The periodontium is a complex tissue consisting of soft and mineralized structures. Several diseases affect the integrity of periodontium causing the destruction of the connective tissue and cells. In recent decades bioactive glass has been used in several studies in the dental field because of its excellent regenerative properties. It is known that the effect of bioactive glass can be enhanced in the nanoscale. In this study we isolated human periodontal dental ligament cells (HPDL) of third molar teeth with orthodontic indication for extraction. Bioactive glass nanoparticles (BGnp) were synthesized by modified Stöber method. The cell viability by Resazurin assay, cell proliferation by 5-Bromo-2-Deoxyuridine (BrdU) assay and (alkaline phosphatase) ALP activity assay were used to evaluate the behavior of HPDL cells in direct contact with particles. It was observed a significant increase in cell viability, cell proliferation and ALP activity in the presence of BGnp when compared to the control group. These results indicate that BGnp induces HPDL cells to proliferate, indicating that it is a potential material to be used for periodontal regeneration through tissue engineering.
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