Human Cancer Biology PI3K/AKT Signaling Is Essential for Communication between Tissue-Infiltrating Mast Cells, Macrophages, and Epithelial Cells in Colitis-Induced Cancer
نویسندگان
چکیده
Purpose: Tounderstand signaling pathways that shape inflamed tissue andpredispose to cancer is critical for effective prevention and therapy for chronic inflammatory diseases.We have explored phosphoinositide 3-kinase (PI3K) activity in human inflammatory bowel diseases and mouse colitis models. Experimental Design: We conducted immunostaining of phosphorylated AKT (pAKT) and unbiased high-throughput image acquisition and quantitative analysis of samples of noninflamed normal colon, colitis, dysplasia, and colorectal cancer. Mechanistic insights were gained from ex vivo studies of cell interactions, the piroxicam/IL-10 / mouse model of progressive colitis, and use of the PI3K inhibitor
منابع مشابه
PI3K/AKT signaling is essential for communication between tissue-infiltrating mast cells, macrophages, and epithelial cells in colitis-induced cancer.
PURPOSE To understand signaling pathways that shape inflamed tissue and predispose to cancer is critical for effective prevention and therapy for chronic inflammatory diseases. We have explored phosphoinositide 3-kinase (PI3K) activity in human inflammatory bowel diseases and mouse colitis models. EXPERIMENTAL DESIGN We conducted immunostaining of phosphorylated AKT (pAKT) and unbiased high-t...
متن کاملLong non-coding RNA FOXO1 inhibits lung cancer cell growth through down-regulating PI3K/AKT signaling pathway
Objective(s): Lung cancer is one of the most common malignant tumors, which seriously threatens the health and life of the people. Recently, a novel long non-coding RNA (lncRNA) termed lncFOXO1 was found and investigated in breast cancer. However, the effect of lncFOXO1 on lung cancer is still ambiguous. The current study aimed to uncover the functions of lncFOXO1 in l...
متن کاملPI3K/Akt inhibition and down-regulation of BCRP re-sensitize MCF7 breast cancer cell line to mitoxantrone chemotherapy
Objective(s):Multidrug resistance (MDR) of cancer cells is a major obstacle to successful chemotherapy. Overexpression of breast cancer resistance protein (BCRP) is one of the major causes of MDR. In addition, it has been shown that PI3K/Akt signaling pathway involves in drug resistance. Therefore, we evaluated the effects of novel approaches including siRNA directed against BCRP and targeted t...
متن کاملBiology of Hepatocellular Carcinoma are Restrained through PI3K/AKt Signaling Pathways and Targeted by Tumor Infiltrating Macrophages
To study the biology of hepatocellular carcinoma are restrained through PI3K/AKt signaling pathways and targeted by tumor infiltrating macrophages.Methods: Mononuclear cells were isolated from healthy adult peripheral blood by density gradient centrifugation and induced with IL 4 for selective activation of macrophagesin vitro.PI3K siRNA and AKT – siRNA were transfected to hepatocellular carcin...
متن کاملInvestigating the role of signaling pathways and cancer stem cells in esophageal cancer with a therapeutic approach
Esophageal cancer (EC) is the sixth main cause of cancer death worldwide. Important genes associated with esophageal cancer include FOXO3, AKT, and GSK3β. Excessive FOXO3 expression inhibits the proliferation of cancer cells. The expression of AKT is involved in controlling cell growth in tumors. GSK3β activity is higher in cancer tissues. Given the effective role of cancer stem cells (CSCs) in...
متن کامل