Two channel amplitude analyser for multi-unit discharges analysis.

نویسنده

Z Tolloczko

چکیده

In neurophysiological experiments, neurmal discharges are often recorded from several units simultaneously with the use of a single micro-electrode. Since it is sometimes desirable to separate the recorded spike potentials with regard to their sources, a number of different types of devices has been designed for that purpose. The apparatus described by Bradley e t al. (1) was provided for selection of units of specific amplitudes from a background of units of various amplitudes and from the noise llevel. However, Schmitt triggars are the input circuits d the device and this aplpeared t o be a disadvantage of this apparatus. Because of the hysteresis effect in Schmitt triggers, some limitations were imposed on the minimum separation distance. The input discriminator (hfferential amplifier) in Landot's instrument (3) has no hysteresis; by means of this device, however, only the spikes of the highest amplitude could be analysed. It. is, of course, also possible to carry out the spike trains selection with the aid of a computer. Dill, hckemann and Naka (2) have ~ e p r t e d about such a possibility, but their method turned out to be expensive. The device described in this paper separates two spike trains recorded simultaneously. One of them may be analysed on line, the second may be stored on a magnetic tape for the future use. Block diagram of the device is shown in Fig. 1, and the complete circuit diagram in Fig. 2. The description of circuit operation follows. The input gate (Q,) leads to two amplitude discriminators. The gate is controlled by the Gate Control Unit 1 (transistors Q, and Q2 and their

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