Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions.

نویسندگان

  • C W Ronson
  • B T Nixon
  • L M Albright
  • F M Ausubel
چکیده

We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

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Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 169 6  شماره 

صفحات  -

تاریخ انتشار 1987