Blood Kinetics and In Vivo Chemotaxis of Transfused Neutrophils : Effect of Collection

To evaluate effect of collection technique infused neutrophils localizing to the skin and short-term storage on in vivo cell funcchamber was 0.1 . 0.06, and 0.006 for tion, neutrophils were collected from 53 unstored PB. IFC, and FL cells, respectively. normal subjects by phlebotomy (PB). interOne-day storage substantially decreased mittent flow centrifugation (IFC), or filtrachamber accumulation of infused neutrotion Ieukopheresis (FL). stored 0 or 1 day. phils. Donor steroid pretreatment had no labeled with 32P-diisopropylfluorophoseffect on chamber results. Thus. in vivo phate. reinfused into the donor. and blood chemotactic ability of IFC neutrophils is kinetics and/or skin chamber accumulation slightly impaired. whereas that of FL cells of labeled cells measured. The blood kineis severely impaired. One-day storage of tics of unstored PB and IFC cells were either cell concentrate causes further cell similar; the kinetics of unstored FL cells damage. were markedly abnormal. The percent of