Molecular Cloning , DNA for Thermostable PectateSequenee , and Expression of the Gene Lyase of Thermophilic BacMus
نویسنده
چکیده
structure with three long leops. The amino acids making up PL 47 are richer in hydrophobic amino acids and glutamic acid than BsPel. The hydropathy profile of PL 47 indicated that the amino acid sequences around putatiye calcium binding sites are more hydrophobic than the same region of BsPel. The gene product expressed in B. subtilts as the host was stable up to 700C and the reaction was optimal around 70eC, as we]1 as native PL 47.
منابع مشابه
Molecular Cloning , DNA for Thermostable PectateSequenee , and Expression of the Gene Lyase of Thermophilic BacMus sp . TSEncoding 47
structure with three long leops. The amino acids making up PL 47 are richer in hydrophobic amino acids and glutamic acid than BsPel. The hydropathy profile of PL 47 indicated that the amino acid sequences around putatiye calcium binding sites are more hydrophobic than the same region of BsPel. The gene product expressed in B. subtilts as the host was stable up to 700C and the reaction was optim...
متن کاملMolecular cloning of Clostridium septicum vaccine strain alpha toxin gene in E. coli
Clostridium septicum a Gram positive anaerobic bacterium produces several toxins including alpha, beta, gamma and delta. C. septicum alpha toxin is lethal and is responsible for a serious disease known as gas gangrene. The aim of the present study was to molecular cloning and sequencing of C. septicum vaccine strain alpha toxin gene. Genomic DNA was extracted using standard phenol and chlorofor...
متن کاملCloning of EprA1 gene of Aeromonas hydrophila in Lactococcus lactis
Bacterial-based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. Developments in genetic engineering have given Gram-positive lacticacid bacteria (LAB) the advantage of being used as a host expression system for antigen delivery to inducethe immune response. A fragment containing the full length of the “eprA1” ...
متن کاملMolecular Cloning, Expression and Enzymatic Assay of Pteridine Reductase 1 from Iranian Lizard Leishmania
Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pterid...
متن کاملMolecular Cloning, Expression and Purification of Protein TB10.4 Secreted by Mycobacterium Tuberculosis
Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, especially in developing countries. One of the main goals in tuberculosis research is to identify antigens which have the ability of inducing cellular and/or humoral immunity in order to use them in diagnostic reagents or vaccine design. The aim of this study was to clone and express the TB'0.4 prot...
متن کامل