Bio011957 1..11
نویسندگان
چکیده
The observation that themembranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography highresolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of
منابع مشابه
Line 806 (06/25/17) -- Metro Rail - Downtown LA - Downtown Santa Monica
— — — 3:26A 3:29A 3:31A 3:35A 3:37A 3:39A 3:41A 3:42A 3:44A 3:48A 3:51A 3:53A 3:55A 3:58A 4:04A 4:06A — — — 3:47 3:50 3:52 3:56 3:58 4:00 4:02 4:03 4:05 4:09 4:12 4:14 4:16 4:19 4:25 4:27 — — — 3:59 4:02 4:04 4:08 4:10 4:12 4:14 4:15 4:17 4:21 4:24 4:26 4:28 4:31 4:37 4:39 — — — 4:11 4:14 4:16 4:20 4:22 4:24 4:26 4:27 4:29 4:33 4:36 4:38 4:40 4:43 4:49 4:51 — — — 4:26 4:29 4:31 4:35 4:37 4:39 4...
متن کاملLine 803 (12/11/16) -- Metro Rail - Metro Green Line - RailEastbound to Norwalk - Westbound to Redondo Beach
— — — — — — — — — — 3:36A 3:42A 3:58A 4:08A — 3:53A 3:55A 3:57A 3:59A 4:02A 4:05A 4:07A 4:09A 4:11A 4:13 4:16 4:21 4:25 — 4:21 4:23 4:25 4:27 4:30 4:33 4:35 4:37 4:39 4:41 4:44 4:49 4:53 4:31A 4:33 4:35 4:37 4:39 4:42 4:45 4:47 4:49 4:51 5:00 5:03 5:08 5:12 4:50 4:52 4:54 4:56 4:58 5:01 5:04 5:06 5:08 5:10 5:12 5:15 5:20 5:24 5:03 5:05 5:07 5:09 5:11 5:14 5:17 5:19 5:21 5:23 5:25 5:28 5:33 5:37...
متن کاملLine 804 (06/26/16) -- Metro Rail - Metro Gold Line (Azusa – Union Station – East LA)
4:20A 4:30A 4:34A 4:40A 4:44A 4:50A 4:56A 4:59A 5:04A 5:05A 5:09A 5:12A 5:17A 5:20A 5:24A 5:28A 5:32A 4:44 4:54 4:58 5:04 5:08 5:14 5:20 5:23 5:28 5:29 5:33 5:36 5:41 5:44 5:48 5:52 5:56 5:04 5:14 5:18 5:24 5:28 5:34 5:40 5:43 5:48 5:49 5:53 5:56 6:01 6:04 6:08 6:12 6:16 5:24 5:34 5:38 5:44 5:48 5:54 6:00 6:03 6:08 6:09 6:13 6:16 6:21 6:24 6:28 6:32 6:36 5:44 5:54 5:58 6:04 6:08 6:14 6:20 6:23 ...
متن کامل11 - Hydroxysteroid dehydrogenase type 1 is a predominant 11 - reductase in the intact perfused rat liver
11 -Hydroxysteroid dehydrogenase type 1 (11 -HSD-1), a regulator of intrahepatocellular glucocorticoid activity, is bidirectional in homogenates but catalyses 11 reduction (regenerating glucocorticoid) in intact primary hepatocytes in culture. To examine this discrepancy at the whole-organ level, we examined 11 -HSD-1 activity in the intact bivascularly perfused rat liver. On a single pass thro...
متن کاملThe generalization of Pascal’s triangle from algebraic point of view
In this paper we generalize Pascal’s Triangle and examine the connections between the generalized triangles and powering integers and polynomials respectively. The interesting and really romantic Pascal’s Triangle is a favourite research field of mathematicians for a very long time. The table of binomial coefficients has been named after Blaise Pascal, a French scientist, but was known already ...
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