Microarray Sub-Grid Detection: A Novel Algorithm (code: SI-ZW)
نویسندگان
چکیده
In this paper, a novel algorithm for detecting microarray sub-grids is proposed. The only input to the proposed algorithm is the raw microarray image, which can be at any resolution, and the subgrid detection is performed with no prior assumptions. The algorithm consists of a series of methods of spot shape detection, spot filtering, spot spacing estimation, and sub-grid shape detection. The algorithm is shown to be successful in dividing images of varying quality into sub-grid regions with no manual interaction. The algorithm is robust against high levels of noise, and high percentages of poorly expressed or missing spots. In addition, the developed algorithm is proven to be effective in locating regular groupings of primitives in a set of non-microarray images, suggesting that the algorithm may have application potential in the general area of image processing.
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