Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli.
نویسندگان
چکیده
BACKGROUND/AIM Biofilm on urinary catheters results in persistent infections that are resistant to antibiotics. In this study, phytochemicals were assessed as alternative antimicrobials in preventing and inactivating E. coli biofilm on urinary catheters. MATERIALS AND METHODS Biofilm prevention was tested using catheter fragments inoculated with E. coli and treated with trans- cinnamaldehyde, p-coumaric, and ferulic acids (0%, 0.1%, 0.25%, and 0.5%) for 0, 1, 3, and 5 days. Inactivation of E. coli biofilm with the same agents at concentrations of 0%, 1%, 1.25%, or 1.5% used for 0, 1, 3, or 5 days was also evaluated. RESULTS All used concentrations of trans-cinnamaldehyde prevented and effectively inactivated E. coli biofilm formed on urinary catheter fragments. p-Coumaric (0.25% and 0.5%) and ferulic acids (0.5%) had preventive action on E. coli biofilm formation in urinary catheter fragments. The number of uropathogenic E. coli cells in biofilm formed in the lumen of a urinary catheter was significantly reduced in the presence of p-coumaric and ferulic acids, but complete inactivation of the biofilm formed was not observed, as opposed to the use of trans-cinnamaldehyde. CONCLUSION The obtained results indicate that phytochemicals maybe an important source of antibiofilm agents that have preventive action on E. coli biofilm formation on urinary catheters.
منابع مشابه
Artificial biosynthesis of phenylpropanoic acids in a tyrosine overproducing Escherichia coli strain
BACKGROUND The phenylpropanoid metabolites are an extremely diverse group of natural products biosynthesized by plants, fungi, and bacteria. Although these compounds are widely used in human health care and nutrition services, their availability is limited by regional variations, and isolation of single compounds from plants is often difficult. Recent advances in synthetic biology and metabolic...
متن کاملEffects of common forage phenolic acids on Escherichia coli O157:H7 viability in bovine feces.
Ruminant animals are carriers of Escherichia coli O157:H7, and the transmission of E. coli O157:H7 from cattle to the environment and to humans is a concern. It is unclear if diet can influence the survivability of E. coli O157:H7 in the gastrointestinal system or in feces in the environment. Feces from cattle fed bromegrass hay or corn silage diets were inoculated with E. coli O157:H7, and the...
متن کاملBiosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli
BACKGROUND Phenylpropanoids are the precursors to a range of important plant metabolites such as the cell wall constituent lignin and the secondary metabolites belonging to the flavonoid/stilbene class of compounds. The latter class of plant natural products has been shown to function in a wide range of biological activities. During the last few years an increasing number of health benefits hav...
متن کاملSynergistic antibiofilm efficacy of a gallotannin 1,2,6-tri-O-galloyl-β-D-glucopyranose from Terminalia chebula fruit in combination with gentamicin and trimethoprim against multidrug resistant uropathogenic Escherichia coli biofilms
In recent years the emergence of multiple drug resistance microbes has become a global public health problem. The aim of the present investigation was to evaluate possible antibiofilm efficacy of a gallotannin 1,2,6-tri-O-galloyl-β-D-glucopyranose from Terminalia chebula fruits alone and in combination with gentamicin and trimethoprim against preformed biofilms of multidrug-resistant (MDR) urop...
متن کاملBiological synthesis of coumarins in Escherichia coli
BACKGROUND Coumarins are a major group of plant secondary metabolites that serves as defense compounds against pathogens. Although coumarins can be obtained from diverse plant sources, the use of microorganisms to synthesize them could be an alternative way to supply building blocks for the synthesis of diverse coumarin derivatives. RESULTS Constructs harboring two genes, F6'H (encoding ferul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Turkish journal of medical sciences
دوره 45 4 شماره
صفحات -
تاریخ انتشار 2015