‘The Polymerase Chain Reaction and Sequence-tagged Sites
نویسنده
چکیده
The polymerase chain reaction (PCR) is an in vitro methocl for selectively amplifying, or synthesizing millions of copies of, a short region of a DNA molecule. The reaction is carried out enzymatically in a test tube and has been successfully applied to regions as small as 100 base pairs and as large as 6000 base pairs. In contrast, DNA cloning is a nonselective in vivo method for replicating DNA fragments within bacterial or yeast cells. Cloned fragments range in length from several hundred to i million base pairs. (See “DNA Libraries” for further discussion of DNA cloning.)
منابع مشابه
Multiplex-Polymerase Chain Reaction for Detecting Microdeletions in The Azoospermia Factor Region of Y Chromosome in Iranian Couples with Non-Obstructive Infertility and Recurrent Pregnancy Loss
Objective Approximately 15 percent of couples are infertile. The male factor is responsible for approximately 50% of the cases. One of the main genetic factors playing a role in male infertility is Y chromosomal microdeletion within the proximal long arm of the Y chromosome (Yq11), named azoospermia factor (AZF) region. Recent studies have also demonstrated that there is a potential connection ...
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