Transfer of oxygen in the glutamine synthetase reaction.

Biochemical and molecular analysis of glutamine synthetase gene in brassica napus L.

Studies on the Reaction Mechanism of Adenosine Triphosphate : Glutamine Synthetase Adenylyl- transferase from Escherichia coli B

Glutamine establishes feedback control of glutamine synthetase (EC 6.3.1.2) in Escherichia coli over a novel loop, viz. by its effect on an enzymatic adenylylation of glutamine synthetase, whose activity is thus modulated. Positive effecters of the adenylyl transfer reaction, magnesium ion and glutamine, increase the number of -SH groups in adenylyltransferase (EC 2.7.7. -) titratable by 5,5’-d...

Regulation of glutamine synthetase. VI. Interactions of inhibitors for Bacillus licheniformis glutamine synthetase.

The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP caused synergistic inhibition of glutamine synthesis. Histidine, alanine, and g...

STUDIES IN VITRO By ANNEMARIE HERZFELD

Two common ways of measuring the potential for glutamine synthesis in a tissue are the rates of formation of y-glutamylhydroxamate either by synthesis from glutamate (the glutamylhydroxamate synthetase reaction) or by transfer from glutamine (the glutamyltransferase reaction); it has not been established, however, that either reaction is a specific measure of glutamine synthetase. By differenti...

Oxidative Modification of Glutamine Synthetase

The first step in the proteolytic degradation of bacterial glutamine synthetase is a mixed function oxidation of one of the 16 histidine residues in the glutamine synthetase subunit (Levine, R. L. (1983) J. Biol. Chem. 258, 11823-11827). A model system, consisting of oxygen, a metal ion, and ascorbic acid, mimics the bacterial system in mediating the oxidative modification of glutamine syntheta...