Chemical morphology of glucan and chitin in the cell wall of the yeast phase of Paracoccidioides brasiliensis.
نویسندگان
چکیده
Short and thick fibers were observed on the outer surface of the yeast phase of Paracoccidioides brasiliensis, and long and thin fibers were seen on the inner surface. The long fibers disappear with chitinase treatment and are composed of chitin. The short fibers disappear under alkali treatment and are composed of alpha-glucan. Comparisons with alpha-(1 --> 3)-glucan isolated from Aspergillus niger and Polyporus betulinus and with chitin from fungal origin support our point of view.
منابع مشابه
Cell wall composition of the yeast and mycelial forms of Paracoccidioides brasiliensis.
Isolation and chemical analyses of the cell walls of the yeast (Y form) and mycelial forms (M form) of Paracoccidioides brasiliensis and Blastomyces dermatitidis revealed that their chemical composition is similar and depends on the form. Lipids, chitin, glucans, and proteins are the main constituents of the cell walls of both forms of these fungi. There is no significant difference in the amou...
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Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America. It is caused by the temperature-dependent dimorphic fungus Paracoccidioides brasiliensis. The P. brasiliensis cell wall is a dynamic outer structure, composed of a network of glycoproteins and polysaccharides, such as chitin, glucan and N-glycosylated proteins. These glycoproteins can interact with the host to affec...
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Paracoccidioides brasiliensis is a dimorphic fungus pathogenic to humans, growing as yeast during infection and as mycelium in the environment. The cell wall of P. brasiliensis consists of chitin and glucan and is involved in pathogenesis (de Agostino Biella et al. 2006). The percentage of chitin in P. brasiliensis ranges from 37-48% in the yeast phase and from 7-18% in the mycelial phase, indi...
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α-1,3-Glucan is present as the outermost layer of the cell wall in the pathogenic yeastlike (Y) form of Paracoccidioides brasiliensis. Based on experimental evidence, this polysaccharide has been proposed as a fungal virulence factor. To degrade α-1,3-glucan and allow remodeling of the cell wall, α-1,3-glucanase is required. Therefore, the study of this enzyme, its encoding gene, and regulatory...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 101 2 شماره
صفحات -
تاریخ انتشار 1970