Sex steroid-opioid interactions associated with the temporal component of avian calling patterns.

Isolation from conspecifics in young, precocial birds predictably induces distress vocalizations (DV) and androgens change this type of vocalization into male typical "crowing" (CR). In addition, opioid peptides are known to exert potent effects on avian vocal behavior. Here we investigate the organizational and activational correlates of sex-steroid actions on opioid-receptor organization and their relevance to the temporal evolution of DV and CR. From the effects of pre- and postnatal steroid applications and postnatal [3H]etorphin binding studies, we find that early steroidal effects become manifested at the behavioral level by changing the characteristic duration of vocalizations. In the male quail this extension of calling duration is accompanied by a clear decrease in opiate binding, whereas in the female there is a moderate increase in binding sites. The transition from DV to CR (within hours) induced by testosterone is correlated with "upregulation" of opiate receptor sites within unilateral brainstem areas of young male quail. Based on these findings, we suggest that organizational steroid effects change the characteristic duration of isolation-induced vocalizations and these effects appear to be manifested at the level of opioid-receptor distribution.