Parametric optimization of feruloyl esterase production from Aspergillus terreus strain GA2 isolated from tropical agro-ecosystems cultivating sweet sorghum.

Studies on Cellulase Production by Solid state Fermentation using Sweet Sorghum bagasse

The present study involves production of cellulolytic enzymes by a newly isolated strain of Aspergillus niger isolated from decaying sugarcane bagasse. Solid-state-fermentation was employed using sweet sorghum bagasse as the substrate for the production of enzyme. One factor at a time approach was used to optimize the process variables like moisture content, pH, temperature, and incubation peri...

Product patterns of a feruloyl esterase from Aspergillus nidulans on large feruloyl-arabino-xylo-oligosaccharides from wheat bran.

A purified feruloyl esterase (EC 3.1.1.73) from Aspergillus nidulans produced in Pichia pastoris was used to study the de-esterification of large feruloyl oligosaccharides consisting of 4 to 20 pentose residues and (xylose plus arabinose) and one ferulic acid residue. The feruloyl oligosaccharides were prepared from total oligosaccharidic hydrolysates from wheat bran treated with a purified end...

Production of Feruloyl Esterase from Aspergillus niger by Solid-State Fermentation on Different Carbon Sources

A mixture of wheat bran with maize bran as a carbon source and addition of (NH(4))SO(4) as nitrogen source was found to significantly increase production of feruloyl esterase (FAE) enzyme compared with wheat bran as a sole carbon and nitrogen source. The optimal conditions in conical flasks were carbon source (30 g) to water 1 : 1, maize bran to wheat bran 1 : 2, (NH(4))SO(4) 1.2 g and MgSO(4) ...

Characteristics and Role of Feruloyl Esterase from Aspergillus Awamori in Japanese Spirits, ‘Awamori’ Production

Design and production in Aspergillus niger of a chimeric protein associating a fungal feruloyl esterase and a clostridial dockerin domain.

A chimeric enzyme associating feruloyl esterase A (FAEA) from Aspergillus niger and dockerin from Clostridium thermocellum was produced in A. niger. A completely truncated form was produced when the dockerin domain was located downstream of the FAEA (FAEA-Doc), whereas no chimeric protein was produced when the bacterial dockerin domain was located upstream of the FAEA (Doc-FAEA). Northern blot ...