Adenine-specific DNA chemical sequencing reaction.
نویسندگان
چکیده
Reaction of DNA with K2PdCl4 at pH 2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCl4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.
منابع مشابه
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ورودعنوان ژورنال:
- Methods in enzymology
دوره 218 شماره
صفحات -
تاریخ انتشار 1987