Efficient determination of diffusion coefficients by monitoring transport during recovery delays in NMR.
نویسندگان
چکیده
A novel NMR approach allows one to efficiently determine translational diffusion coefficients of macromolecules in solution. This method for Signal Optimization with Recovery in Diffusion Delays (SORDID) monitors transport occurring during the recovery times between consecutive scans so that the duration of the measurements can be reduced approximately by a factor two.
منابع مشابه
Efficient determination of diffusion coefficients by monitoring transport during recovery delays in NMRw
Nuclear magnetic resonance spectroscopy (NMR) provides a method of choice to probe transport phenomena on a microscopic scale. In liquid phase, it is possible to explore translational displacements of molecules due to diffusion or flow. Although diffusion can be determined by other methods, pulsed field gradient (PFG) techniques are not invasive and offer a remarkable degree of detail both in v...
متن کاملMethods to determine slow diffusion coefficients of biomolecules: applications to Engrailed 2, a partially disordered protein.
We present new NMR methods to measure slow translational diffusion coefficients of biomolecules. Like the heteronuclear stimulated echo experiment (XSTE), these new methods rely on the storage of information about spatial localization during the diffusion delay as longitudinal polarization of nuclei with long T(1) such as nitrogen-15. The new BEST-XSTE sequence combines features of Band-selecti...
متن کاملDetermination of gas-diffusion and interface-mass-transfer coefficients in fracture-heavy oil saturated porous matrix system
For the modeling and simulation of oil recovery from naturally fractured reservoirs during the gas injection process, an accurate value of the molecular diffusion coefficient (MDC) of gases from a gas filled fracture into the oil matrix is essential. During the injection of miscible fluids with oil, transport of the injectant and oil are controlled by fracture and matrix properties. Diffusion i...
متن کاملDeuterium stimulated-echo-type PGSE NMR experiments for measuring diffusion: application to a liquid crystal.
The accessibility of molecular self-diffusion coefficients in anisotropic materials, such as liquid crystals or solids, by stimulated-echo-type (2)H PGSE NMR is examined. The amplitude and phase modulation of the signal in the stimulated-echo-type sequence by the static quadrupole coupling during the encoding/decoding delays is suppressed by adjusting the pulse flip angles and the phase cycle. ...
متن کاملIncreasing the sensitivity of NMR diffusion measurements by paramagnetic longitudinal relaxation enhancement, with application to ribosome–nascent chain complexes
The translational diffusion of macromolecules can be examined non-invasively by stimulated echo (STE) NMR experiments to accurately determine their molecular sizes. These measurements can be important probes of intermolecular interactions and protein folding and unfolding, and are crucial in monitoring the integrity of large macromolecular assemblies such as ribosome-nascent chain complexes (RN...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 48 43 شماره
صفحات -
تاریخ انتشار 2012