Development and application of an analytical method using gas chromatography/triple quadrupole mass spectrometry for characterizing alkylated chrysenes in crude oil samples.

RATIONALE Recent advances in analytical techniques have led to the development of gas chromatography/triple quadrupole mass spectrometry methods that allow the identification of target analytes in complex environmental samples. We have employed this technology to develop a method for characterizing alkylated chrysenes, which are environmental toxins that are resistant to weathering. METHODS An Agilent 7890 gas chromatograph coupled to an Agilent 7000B triple quadrupole mass spectrometer was used. The mass spectral fragmentation of seven commercially available alkylated chrysene standards was studied under full-scan and product-ion scan conditions. The calibration curves used were in the linear range with r(2) values greater than 0.99. The recovery and limit of detection of target analytes in the samples were in the range of 80-120% and 0.11-1.09 ng/mL, respectively. RESULTS The information inferred from full-scan and product-ion scan data was combined with literature data to develop a GC/MS/MS method for the identification and quantification of C1 -, C2 -, C3 -, and C4 -chrysene homologues. The method was employed to characterize MC252 crude oil which was released during the 2010 Deepwater Horizon accident. The results showed that the chrysene concentrations estimated by the proposed method were well within the range of previously reported values. CONCLUSIONS The proposed method is useful for analyzing chrysene and its alkylated homologues in crude oil samples.