DNA Integrity Number (DIN) For the Assessment of Genomic DNA Samples in Real-Time Quantitative PCR (qPCR) Experiments
نویسنده
چکیده
Real-time quantitative PCR (qPCR) is an indispensable molecular biology tool in the post-genome era. QPCR assays have become a robust and ideal tool for several applications such as clinical diagnostics, gene expression studies, biomarker discovery, and more. Using an intact genomic DNA (gDNA) template is crucial for generating meaningful qPCR data. Therefore, assessment of the gDNA integrity is important. With the Agilent 2200 TapeStation system, the Agilent Genomic DNA ScreenTape assay, and the DNA Integrity Number (DIN)1, the assessment of gDNA integrity has become easy, reliable, and objective. In this study, we demonstrate the application of the Genomic DNA ScreenTape assay to assess the integrity of gDNA samples before qPCR reactions. The impact of gDNA sample integrity on target amplifi cation, quantitation cycle (Cq), and target quantitation is assessed using DIN as a QC criteria.
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