Immunologic identification of M. leprae. Immunofluorescence and complement fixation.
نویسندگان
چکیده
A markedly improved immunofluorescent technic employing FITC conjugated IgG antibody prepared from lepromatous serum is described as a means of specific identification of M. leprae. An additional immunologic identification method for M. leprae is presented as a micro-complement fixation technic employing antigen rather than antibody dilution. Studies with these technics suggest that M. leprae specific antigen is probably a surface antigen and has as part of its mosaic a lecithin-phospholipid component. It is not unlikely that it is a protein-glyco-phospholipid with a polysaccharide component. These technics employed with nodular extract (NE) from lepromas, with 15 strains of mycobacteria, with M. leprae from human tissue as well as from previously reported in vitro culture, strongly reinforce the allegation that M. leprae is readily cultivated in vitro in the hyaluronic acid based medium LA-3, previously reported.
منابع مشابه
Phenolic glycolipid-1 of Mycobacterium leprae binds complement component C3 in serum and mediates phagocytosis by human monocytes
Previous studies from this laboratory have demonstrated that Mycobacterium leprae, an obligate intracellular bacterial parasite, enters human mononuclear phagocytes via complement receptors on these host cells and bacterium-bound C3. The present study investigates the role of M. leprae surface molecules in C3 fixation and phagocytosis. By enzyme-linked immunosorbent assay, C3 binds selectively ...
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ورودعنوان ژورنال:
- International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
دوره 44 3 شماره
صفحات -
تاریخ انتشار 1976