Mice by Polyadenylic Acid: Polyuridylic Acid Complex
نویسندگان
چکیده
The effect of polyadenylic acid: polyuridylic acid complex (poly A: U) on immunization was investigated in mice vaccinated by killed Brucella melitensis cells suspended in incomplete adjuvant or in saline. Addition of 300 ,ug of poly A:U to vaccines rendered 3 X 108 B. melitensis cells in saline as immunogenic as 3 X 10"1 cells in oil adjuvant against a severe B. abortus challenge. In the mouse and Brucella system, poly A:U exerts an adjuvant effect on immunity but does not stimulate production of circulating antibodies that could be demonstrated at the time of autopsy.
منابع مشابه
Inhibition of C3H/He mouse mammary tumor growth by combined treatment with cyclophosphamide and polyadenylic-polyuridylic acid.
The antitumor effect of an immunomodulator, polyadenylic-polyuridylic acid, in combination with cyclophosphamide (CY) was studied in C3H/He mice bearing established mammary tumors. On Day 14 after tumor graft, mice received either CY (90 mg/kg) alone every 2 weeks for a total of four inoculations or alternate weekly inoculations of the same dose of CY and polyadenylic-polyuridylic acid (300 mic...
متن کاملStimulation of antibacterial vaccination in mice by polyadenylic acid: polyuridylic acid complex.
The effect of polyadenylic acid:polyuridylic acid complex (poly A:U) on immunization was investigated in mice vaccinated by killed Brucella melitensis cells suspended in incomplete adjuvant or in saline. Addition of 300 mug of poly A:U to vaccines rendered 3 x 10(8)B. melitensis cells in saline as immunogenic as 3 x 10(11) cells in oil adjuvant against a severe B. abortus challenge. In the mous...
متن کاملThe mitogenic activity of polyadenylic-polyuridyclic acid complexes.
Polyadenylic-polyuridylic acid complexes (poly A:U) at the 1-5 mu g level, were mitogenic for spleen cells when given intravenously to normal Balb or cortisone-treated mice. Similarly, mitogenicity was evident when poly A:U was added to tissue culture fluids containing spleen cells from normal or cortisone-treated mice, or bone marrow cells from normal mice. Under these conditions, this adjuvan...
متن کاملProtein synthesis in a cell-free extract from Staphylococcus aureus.
Cell-free Staphylococcus aureus extracts have been prepared which actively incorporate amino acids into protein. The requirements for amino acid incorporation of this preparation were strongly suggestive of de novo protein synthesis, since it showed an absolute requirement for ribosomes, 105,000 x g supernatant fluid, energy source, and magnesium ion. The stability of these extracts was greatly...
متن کاملThe interaction of aflatoxin B1 with polynucleotides and its effect on ribonucleic acid polymerase.
1. The interaction of aflatoxin B(1) with different polynucleotides was studied spectrophotometrically. Equations were derived that enable the degree of binding to be determined without first determining the extinction coefficient of the bound form. 2. The interaction with calf thymus DNA obeys first-order relationships with an association constant of 0.40mm(-1), but there is some evidence for ...
متن کامل