Isolation and characterization of 17 polymorphic microsatellite loci for the widespread ascidian Didemnum perlucidum (Tunicata, Ascidiacea)
نویسندگان
چکیده
The colonial tunicate Didemnum perlucidum has been identified from numerous tropical and temperate locations worldwide, but its native and introduced ranges remain largely unknown. In Australia, D. perlucidum is listed as a target species under the introduced marine pest national monitoring network. In order to investigate the introduced status and potential routes of introduction and dispersal of D. perlucidum, we developed 17 new polymorphic microsatellite markers using 454 shotgun sequencing. Two to six alleles per locus were detected. No evidence of linkage disequilibrium between pairs of loci was identified and 12 of the 17 loci were in Hardy-Weinberg equilibrium.
منابع مشابه
The role of Didemnum perlucidum F. Monniot, 1983 (Tunicata, Ascidiacea) in a marine fouling community
Didemnum perlucidum is a colonial ascidian that is cryptogenic in southern Brazil but introduced in many regions around the world. It is common in ports and marinas where it may form large colonies although its role in fouling communities is poorly understood. The goal of this study was to evaluate the role of D. perlucidum in a marine fouling community comprised mainly of non-indigenous specie...
متن کاملIdentification of Ascidians (Tunicata: Ascidiacea) around Larak Island in the Persian Gulf
Despite the variety of marine organisms recorded from the Persian Gulf, acidians located along the Persian Gulf have not received much attention. We collected Ascidian samples from four sites along Larak Island (Persian Gulf) using Scuba diving and snorkeling at a depth that ranged from 5-15 m. Taxonomic identification was carried out by authoritative keys and texts. We found 4 species that be...
متن کاملCytotoxicity of the crude extracts of Marine ascidians (Tunicata: Ascidiacea) from Tuticorin, Southeast coast of India
Marine organisms such as ascidians, sponges and soft corals, containing symbiotic microorganisms are a rich source of bioactive compounds. This study examined the cytotoxic potential of two ascidians found in the coastal waters of Tuticorin. The ascidians samples Didemnum psammatode and Phallusia arabica were extracted in methanol and ethanol. The crude extracts were tested for cytotoxicity usi...
متن کاملIsolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...
متن کاملIsolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...
متن کامل