Isolation of plasmids from E. coli by alkaline lysis.
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Exploring PFGE for Detecting Large Plasmids in Campylobacter jejuni and Campylobacter coli Isolated from Various Retail Meats
Campylobacter spp. is one of the most prevalent bacterial pathogens in retail meat, particularly poultry, and is a leading cause of diarrhea in humans. Studies related to Campylobacter large plasmids are limited in the literature possibly due to difficulty in isolating them using available alkaline lysis methods. The objectives of this study were to determine the prevalence of plasmids, particu...
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Isolation of highly purified plasmid DNA from E. coli is an essential step in many techniques of current molecular biology. Traditional techniques for plasmid isolation are the alkaline lysis technique of Birnboim and Doly (2) and the boiling method of Holmes and Quigley (4), which are normally followed by a further purification by cesium chloride gradient centrifugation (9). Though the purity ...
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 235 شماره
صفحات -
تاریخ انتشار 2003