a,-Macroglobulin-Kallikrein Complexes Detect Contact System Activation in Hereditary Angioedema and Human Sepsis
نویسندگان
چکیده
Activation of the contact system has been documented in severe sepsis and hereditary angioedema, but a sensitive, specific, and quantitative assay for assessing the degree of involvement of this proteolytic enzyme cascade is not yet available. We have developed a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the %macroglobulin-kallikrein (qM-Kal) complex using an F(ab'), derivative of a monospecific polyclonal antibody against c+M as the capture antibody and a unique murine monoclonal antibody, 13Gl1, against the heavy chain of kallikrein as the detector antibody. The assay does not detect complexes in normal plasma but reacts with complexes generated by activating normal plasma with dextran sulfate at 4°C in a range of 5 to 375 nmol/L. A close correlation of the ELISA with an amidolytic assay for C+M-Kal was documented.
منابع مشابه
Alpha 2-macroglobulin-kallikrein complexes detect contact system activation in hereditary angioedema and human sepsis.
Activation of the contact system has been documented in severe sepsis and hereditary angioedema, but a sensitive, specific, and quantitative assay for assessing the degree of involvement of this proteolytic enzyme cascade is not yet available. We have developed a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the alpha 2-macroglobulin-kallikrein (alpha 2M-Kal) complex using...
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