Biological Applications of Microplate Scintillation Counting using the TopCount

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The microplate format has become increasingly popular in biological experimentation due to its small size, easy sample handling, increased throughput, decreased sample preparation, and small sample volume. The need to perform increasing numbers of assays has sparked interest in high throughput, microplate compatible radioisotope counters. Many of these biological experiments are performed on glass fiber filters or synthetic membranes. These media may be used to separate specific components of an assay mixture for counting, or target substances may be immobilized for analysis with radiolabeled or luminescent probes. The diversity of assays requires a number of different filter types and sizes. We have developed a microplate scintillation and luminescence counter and associated accessories with which to carry out many of these procedures in several popular formats. Described in this paper are some experiments that have been conducted to demonstrate the feasibility of using microplatebased instrumentation to carry out several classes of important biological assays. Biological Applications of Microplate Scintillation Counting using the TopCount C

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تاریخ انتشار 2004