Molecular Basis of Phosphatidylinositol Mannoside Biosynthesis and Regulation in Mycobacteria*
نویسندگان
چکیده
Marcelo E. Guerin, Jana Korduláková, Pedro M. Alzari, Patrick J. Brennan and Mary Jackson From the Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Cientificas Universidad del País Vasco/Euskal Herriko Unibertsitatea (CSIC-UPV/EHU), Barrio Sarriena s/n, Leioa, Bizkaia, 48940, Spain, Departamento de Bioquímica, Universidad del País Vasco, Spain IKERBASQUE, Basque Foundation for Science, 48011, Bilbao, Spain, Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 84215 Bratislava, Slovakia, Unité de Biochimie Structurale (CNRS URA 2185), Institut Pasteur, 25 rue du Dr. Roux, 75724, Paris Cedex 15, France, and Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, 80523-1682, USA.
منابع مشابه
Definition of the first mannosylation step in phosphatidylinositol mannoside synthesis. PimA is essential for growth of mycobacteria.
We examined the function of the pimA (Rv2610c) gene, located in the vicinity of the phosphatidylinositol synthase gene in the genomes of Mycobacterium tuberculosis and Mycobacterium smegmatis, which encodes a putative mannosyltransferase involved in the early steps of phosphatidylinositol mannoside synthesis. A cell-free assay was developed in which membranes from M. smegmatis overexpressing th...
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Mycobacterium tuberculosis and related members of the genus Mycobacterium contain a number of inositol-based lipids, such as phosphatidylinositol mannosides, lipomannan and lipoarabinomannan. The synthesis of phosphatidylinositol in M. smegmatis is essential for growth and myo-inositol is a key metabolite for mycobacteria. Little is known about the biosynthesis of inositol in mycobacteria and t...
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