A mathematical model for selective differentiation of neural progenitor cells on micropatterned polymer substrates.

Directed growth and selective differentiation of neural progenitor cells on micropatterned polymer substrates.

Directional growth and differentiation of adult rat hippocampal progenitor cells (AHPCs) were investigated on micropatterned polymer substrates in vitro. Astrocytes or AHPCs cultured on micropatterned polystyrene substrates chemically modified with laminin exhibited over 75% alignment in the groove direction. AHPCs co-cultured with astrocytes preferentially acquired neuronal morphology, with ne...

A mathematical model for IL6-induced differentiation of neural progenitor cells on a micropatterned polymer substrate

Astrocyte-derived soluble factors promoting neuronal differentiation of adult neural progenitor cells

.........................................................................................................v CHAPTER 1. GENERAL INTRODUCTION Introduction ..........................................................................................................1 Dissertation Organization .....................................................................................2 Abbreviations .............

Oriented astroglial cell growth on micropatterned polystyrene substrates.

In an effort to develop a permissive environment for neural stem cell differentiation, directional growth of astrocytes has been achieved on polymer substrates in vitro. Manipulating a combination of physical and chemical cues, astrocyte adhesion and alignment in vitro were examined. To provide physical guidance, micropatterned polymer substrates of polystyrene (PS) were fabricated. Laminin was...

A New Two Step Induction Protocol for Neural Differentiation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells

Background: In this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. Materials and Methods: Human umbilical cord blood-derived mesenchymal stem cells were first cultured in Dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incu...