Evaluation of the Performances of Ribosomal Database Project (RDP) Classifier for Taxonomic Assignment of 16S rRNA Metabarcoding Sequences Generated from Illumina-Solexa NGS

نویسندگان

  • Giovanni Bacci
  • Alessia Bani
  • Marco Bazzicalupo
  • Maria Teresa Ceccherini
  • Marco Galardini
  • Paolo Nannipieri
  • Giacomo Pietramellara
  • Alessio Mengoni
چکیده

Here we report a benchmark of the effect of bootstrap cut-off values of the RDP Classifier tool in terms of data retention along the different taxonomic ranks by using Illumina reads. Results provide guidelines for planning sequencing depths and selection of bootstrap cut-off in taxonomic assignments.

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عنوان ژورنال:

دوره 3  شماره 

صفحات  -

تاریخ انتشار 2015