Microencapsulation of Cells
نویسنده
چکیده
................................................................................................. 2 Introduction Hormone replacement............................................................................ 3 Encapsulation of cells........................................................................ 4 Problem statement..................................................................................... 6 Biocompatibility.............................................................................. 6 Immunoprotection............................................................................. 6 Encapsulated cell necrosis................................................................... 7 Design specifications.................................................................................. 7 Biomaterials for cell encapsulation.................................................................. 8 Alginate/poly-L-lysine/alginate (APA).................................................... 8 Hyaluronic acid (HA)........................................................................ 10 Polyethylene glycol (PEG)................................................................... 11 Past work................................................................................................ 12 Current design PEG biomaterial............................................................................... 13 Crosslinking scheme.......................................................................... 14 Mouse MA-10 Leydig tumor cell line...................................................... 15 Microfluidic capsule generation............................................................ 17 Current experiments Crosslinking studies........................................................................... 19 Viability assays................................................................................ 19 Microfluidic operation and cell encapsulation............................................ 20 Cost analysis............................................................................................ 23 Future work............................................................................................. 23 Conclusion.............................................................................................. 25 References............................................................................................... 26 Appendix: Product Design Specifications.......................................................... 28
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