Beyond library size : a field guide to NGS normalization

Beyond library size : a field guide to NGS normalization

Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard

Normalization of human RNA-seq experiments employing chimpanzee RNA as a spike-in standard is reported. Human and chimpanzee RNAs exhibit single nucleotide variations (SNVs) in average 210-bp intervals. Spike-in chimpanzee RNA would behave the same as the human counterparts during the whole NGS procedures owing to the high sequence similarity. After discrimination of species origins of the NGS ...

New challenges for Adaptive Optics: Extremely Large Telescopes

The performance of an adaptive optics (AO) system on a 100 m diameter ground based telescope working in the visible range of the spectrum is computed using an analytical approach. The target Strehl ratio of 60 % is achieved at 0.5 μm with a limiting magnitude of the AO guide source near R∼ 10, at the cost of an extremely low sky coverage. To alleviate this problem, the concept of tomographic wa...

Optimization of genomic DNA shearing by sonication for next-generation sequencing library preparation

* Author for correspondence: Dr. Maha Abdullah, Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia. Tel.: 603-89472375, Fax: 603-89412787, Email: [email protected]. Abstract. Next-generation sequencing (NGS) technologies, capable of sequencing genomic DNA and RNA at high throughput with unprecedented speed, hav...

Optimized NGS Library Preparation with Acoustic Liquid Handling APPLICATION NOTE

Radiant Genomics and Labcyte have developed a low-volume, multiplexed next-generation sequencing (NGS) library preparation method using the Echo® 525 Liquid Handler. In addition to cost savings, we demonstrate an increase in sample-processing throughput with the Echo system due to efficient multiplexing, which would otherwise remain impractical using standard liquid handling automation. In addi...