Rapid detection of methicillin-sensitive and resistanct Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococci from blood cultures by automated PCR assay
نویسندگان
چکیده
Introduction / objectives We assessed the usefulness of a new automated molecular assay (GenomEra MRSA Confirm, Abacus Diagnostica Oy, Finland), which amplifies simultaneously the marker of methicillin resistance (mecA) and a Staphylococcus aureus-specific DNA segment, in detection of methicillin sensitive and resistant S. aureus (MSSA and MRSA) and other methicillin resistant staphylococci (MRCoNS) in blood culture samples.
منابع مشابه
One-step sample preparation of positive blood cultures for the direct detection of methicillin-sensitive and -resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci within one hour using the automated GenomEra CDXTM PCR system
A method for the rapid detection of methicillinsensitive and -resistant Staphylococcus aureus (MSSA and MRSA, respectively) and methicillin-resistant coagulasenegative staphylococci (MRCoNS) with a straightforward sample preparation protocol of blood cultures using an automated homogeneous polymerase chain reaction (PCR) assay, the GenomEraTM MRSA/SA (Abacus Diagnostica Oy, Turku, Finland), is ...
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Nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. The aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. The presence or absence of the methicillin-resistance gene in 115 clinical isolates of Staphylococcus aureus and 50 isolates of Coagulase Negative Staphy...
متن کاملردیابی ژن ایجاد مقاومت نسبت به متی سیلین (mec-A) در عفونتهای چرکی ناشی از استافیلوکوک بوسیله PCR
The emergence of Staphylococcus strains resistant to methicillin occurred shortly after the introduction of methicillin into clinical use . Resistance to methicillin in clinical isolates of Staphylococci has been explained by the production of a characteristic penicillin-binding protein (PBP) , designated PBP2a, that has a decrea...
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Major challenges in diagnostic molecular microbiology are to develop a simple assay to distinguish Staphylococcus aureus from the less virulent but clinically important coagulase-negative staphylococci (CoNS) and to simultaneously determine their antibiotic resistance profiles. Multiplex PCR assays have been developed for the detection of methicillin- and mupirocin-resistant S. aureus and CoNS ...
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