Creatine phosphokinase in mammalian brain.

Association of creatine phosphokinase with the cytoskeleton of cultured mammalian cells

Using an antibody specific for creatine phosphokinase (CPK), we have discovered an association between that enzyme and the cytoskeleton. Immunofluorescence observations show that CPK is associated with intermediate filaments in PTK cells and BALB/3T3 cells. The CPK distribution also follows intermediate filaments when cells are treated with colchicine.

Assessment of The Diagnostic Capability of Serum Creatine Phosphokinase and Its Isoenzyme in Ectopic Pregnancy

Creatine phosphokinase values and myalgia during isotretinoin therapy

Background: Systemic isotretinoin is a very effective medication for the treatment of acne, but it has some side effects. One of its side effects is musculoskeletal problems such as increased levels of creatine phosphokinase (CPK), myalgia, and serious muscle damage such as rhabdomyolysis. The aim of this study was to evaluate the incidence of myalgia and its relationship with the elevation of ...

The relationship between intracompartment pressure with symptoms of compartment syndrome and the level of creatine phosphokinase in limb trauma

Background: Acute compartment syndrome is considered a debilitating complication of limb trauma. Early detection of this compartment syndrome helps us in the early initiation of treatment which will result in preventing its subsequent complications reported in these cases. Methods: This research was an analytical cross-sectional study. Patients with direct trauma to extremities, who were refer...

Kinetic properties and the functional role of particulate MM-isoenzyme of creatine phosphokinase bound to heart muscle myofibrils.

Fractional extractions of heart muscle have revealed that about 30% of cellular creatine phosphokinase activity are located in mitochondria and about 20% are bound to myofibrils [ 1,2] . The presence of significant creatine phosphokinase activity in heart and skeletal muscle myofibrils has been demonstrated also in studies of isolated myofibrillar preparations [ 1,3,4] . This particulate enzyme...

Release of enzymes from adult rat heart myocytes.

The release of lactic dehydrogenase, creatine phosphokinase, and aspartate aminotransferase from initially viable, metabolically competent adult rat heart myocytes has been examined. Freshly isolated cells contain levels of total lactic dehydrogenase, creatine phosphokinase, and aspartate aminotransferase, as well as lactic dehydrogenase and creatine phosphokinase isoenzyme profiles that are qu...