USE OF TASSEL-SEED (Ts-S) MAIZE FOR ASSIMILATE TRANSPORT STUDIES USING INTACT OR DETACHED TASSEL BRANCHES
نویسندگان
چکیده
The anatomy of normal maize (Zea mays L.) ears precludes easy access to the kernel pedicel tissue for in vivo assimilate transport experiments. We investigated the possibility of using the Tassel·seed (Ts·51 mutant of maize as a model system for studies of assimilate movement into developing maize kernels. Hypodermic needles (26 gauge, 9.5 mm long) inserted under the pedicel epidermis of intact plants were used to introduce solutes into the pedicel free space. Addition· ally, excised tassel branches were incubated in buffered solutions con· taining 100 mM 14C·labeled sugars. The movement of apoplastic and symplastic fluorescent dyes as well as 14C·labeled assimilates and in· cubation solutions showed that Ts-5 maize provides a useful experimental system for study of the mechanism of assimilate transport into
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