Folate derivatives in the liver of riboflavin--deficient rats.
نویسنده
چکیده
In a preceding paper of ours,1 it was reported that a marked decrease of N5,10methylenetetrahydrofolate reductase as well as a considerable decrease of N5 methyltetrahydrofolate transferase activity in the liver was found in riboflavin deficient rats. In the present study, chromatographic analysis followed by microbiologic assay of folate derivatives in the liver was made on rats with or without riboflavin deficiency. The results revealed that there was a tendency of accumulation of folate derivatives other than N5-methyltetrahydrofolic acid in the liver of riboflavin defi cient rats shortly after an intramuscular injection of folic acid.
منابع مشابه
Application of Riboflavin-Deficient Rat Erythrocytes in the in Vitro Assay of Faba Bean Toxicity
Background: Faba bean toxicity (favism) is a common condition observed in our region. Objective: To develop a short and simple technique involving the use of riboflavin-deficient rats to test in vivo faba bean toxicity. Methods/Results: Sprague Dawley rats were maintained on a riboflavin-deficient diet and their vitamin B2 status was monitored by the assay of erythrocyte glutathione reductase (...
متن کاملThe effect of riboflavin deficiency on methylenetetrahydrofolate reductase (NADPH)( EC 1.5.1.20) and folate metabolism in the rat
1. Riboflavin deficiency at two levels of severity was produced in weanling rats by feeding deficient diets for 6 weeks and using neck collars to prevent coprophagy. The severity of deficiency was monitored by growth, liver flavin levels and the activation coefficient of erythrocyte glutathione oxidoreductase (NAD(P)H) (EC 1 . 6 .4 .2 ) . Control groups, receiving the same diet with ample added...
متن کاملThe effect of riboflavin deficiency on methylenetetrahydrofolate reductase (NADPH) (EC 1.5.1.20) and folate metabolism in the rat.
1. Riboflavin deficiency at two levels of severity was produced in weanling rats by feeding deficient diets for 6 weeks and using neck collars to prevent coprophagy. The severity of deficiency was monitored by growth, liver flavin levels and the activation coefficient of erythrocyte glutathione oxidoreductase (NAD(P)H) (EC 1.6.4.2). Control groups, receiving the same diet with ample added ribof...
متن کاملEffects of riboflavin deficiency upon concentrations of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in Novikoff hepatoma in rats.
The concentrations of riboflavin and of the two coenzymes derived from riboflavin, flavin mononucleotide and flavin adenine dinucleotide (FAD), were determined in Novikoff hepatoma grown i.p. and in host liver of normal and riboflavin-deficient male Holtzman rats. Riboflavin deficiency significantly prolonged the 50% survival time of tumor-bearing animals from 5.7 to 10.8 days. The growth of No...
متن کاملEffect of protein depletion and subsequent repletion on in vivo incorporation of ( 14 C) amino acid into rat liver proteins in riboflavin deficiency.
The effect of riboflavin deficiency on in vivo incorporation of [1-14C] leucine into proteins of liver has been studied on rats following protein depletion and subsequent repletion. The increase in the incorporation of [14C] amino acid into liver proteins follow ing protein depletion was less marked in riboflavin-deficient rats. But repletion with 40% protein demonstrated marked diminished prot...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Tohoku journal of experimental medicine
دوره 95 1 شماره
صفحات -
تاریخ انتشار 1968