Thermostabilization of Firefly Luciferases Using Genetic Engineering
نویسندگان
چکیده
منابع مشابه
Thermostabilization of firefly luciferase by in vivo directed evolution.
Firefly luciferase is widely used in a number of areas of biotechnology and molecular biology. However, rapid inactivation of wild-type (WT) luciferases at elevated temperatures often hampers their application. A simple non-lethal in vivo screening scheme was used to identify thermostable mutants of luciferase in Escherichia coli colonies. This scheme allowed carrying out each cycle of mutagene...
متن کاملA Quaternion Firefly Algorithm to Solve a Multi-row Facility Layout Problem (RESEARCH NOTE)
In this paper, a quaternion firefly algorithm is utilized to solve a multi-row facility layout design problem with the objective of minimizing the total cost of transportation. A quaternion firefly algorithm takes the motion of the firefly as a quaternion one. Consequently, the solution space is explored more accurately and the answers are of higher quality. That is, the answers are considerabl...
متن کاملExpression and Purification of the luciferase enzyme and in Vivo ATP Assay
Introduction: Gene expression and purification of luciferases from the firefly, Lampyris turkestanicus, and optimization of cellular ATP measurements were performed. Methods: cDNA encoding luciferases from Lampyris turkestanicus was transferred from pQE30 vector into pET28a expression vector and pLtu28 was built. Newly constructed vector was expressed in E. coli XL1 Blue and the recombinant l...
متن کاملApproaches to engineer stability of beetle luciferases
Luciferase enzymes from fireflies and other beetles have many important applications in molecular biology, biotechnology, analytical chemistry and several other areas. Many novel beetle luciferases with promising properties have been reported in the recent years. However, actual and potential applications of wild-type beetle luciferases are often limited by insufficient stability or decrease in...
متن کاملDynamic Replication based on Firefly Algorithm in Data Grid
In data grid, using reservation is accepted to provide scheduling and service quality. Users need to have an access to the stored data in geographical environment, which can be solved by using replication, and an action taken to reach certainty. As a result, users are directed toward the nearest version to access information. The most important point is to know in which sites and distributed sy...
متن کامل