464 Specific Venom Ige Decrease during 5 Years of Venom Immunotherapy (VIT): Clinical Relevance in Stung and Not Stung Patients

deficiency and allergies. Methods: Clinical and laboratory tests were conducted on 579 potentially healthy people (172 women and 407 men, between 25 and 30 years), performed twice: in a diagnosis and after 6 to 12 months. Lymphocytes and NK cells from peripheral blood were assessed using cytometry. G, A, M immunoglobulin concentrations were determined by the turbidimetry. IgE concentration was measured by ELFA. HSV2, HIV and Chlamydia trachomatis infections were detected on the basis of presence of IgM and IgG antibodies tested by ELISA. For the assessment of HPV infections, DNA from the urinary-tract, squamous, and epithelial cells was tested through PCR. The statistical analysis was undertaken using regression analyses. Results: In 579 people in the case of 65 people HPV infections were confirmed 10% men, 14.8% women. In 65 patients, in 1 case concentration of IgA was decreased, in 7 cases IgM. In 21 cases, there were higher concentrations of IgE. During the cytometric analyses a decreased number of T CD31 was found in 3 cases, BCD191 in 5, TCD41 in 5, T CD81 in 4, and NK cells in 8 cases. The check for HSV2 was positive for 17 people: 1.5% men, 6.4% women. In this group in 3 cases the concentration of IgA was decreased; in 2 cases IgM was lowered, in 4 cases there were higher concentrations of IgE. Decreased number of lymphocytes CD191 was observed for 1 person and for 1 person NK cells. Chlamydia trachomatis infections were positive in 10 cases: 1.5% men, 2.3% women; in this group 1 person had lower IgA and 2 persons had increased concentrations of IgE. HIV infection was negative for all the groups. The examination was repeated for 171 out of 579 people after 6 months. HPV infections were positive in 12.9% cases: 14.9% men, 10.8% women; HSV2 in 11 persons: 2.3% men, 10.7% women; Chlamydia trachomatis in 4 cases: 2.3% men 2.4% women. Conclusions: No significant correlation was observed between HPV, HSV2, HIV and Chlamydia trachomatis infections and a relevant deviation from the norm of the investigated immunological parameters.