Characterization of heterotrimeric G-proteins in adult Acanthocheilonema viteae.

Essential role of chitinase in the development of the filarial nematode Acanthocheilonema viteae.

Chitinases of pathogens have been proposed as potential targets of vaccines or specific inhibitors. We studied the genomic organization, transcript levels, developmental expression, and biological function of chitinases in the rodent filarial nematode Acanthocheilonema viteae, a model organism for human-pathogenic filarial worms. Characterization of nine genomic clones from an A. viteae phage l...

Developmental and functional characterization of cystatin and chitinase of Acanthocheilonema viteae

Receptor usage by the Acanthocheilonema viteae-derived immunomodulator, ES-62.

ES-62 is an immunomodulatory phosphorylcholine (PC)-containing glycoprotein secreted by the rodent filarial nematode Acanthocheilonema viteae. Previously, the use of knockout mice has revealed the effects of ES-62 on macrophages and dendritic cells to be dependent on TLR4. However, it is possible that ES-62 may interact with additional proteins on the surfaces of target cells and hence that cel...

Brugia malayi and Acanthocheilonema viteae: antifilarial activity of transglutaminase inhibitors in vitro.

The possible involvement of transglutaminase-catalyzed reactions in survival of adult worms, microfilariae (mf), and infective larvae of the filarial parasite Brugia malayi was studied in vitro by using the specific pseudosubstrate monodansylcadaverine (MDC) and the active-site inhibitors cystamine or iodoacetamide. These inhibitors significantly inhibited parasite mobility in a dose-dependent ...

Molecular and immunological characterisation of Acanthocheilonema viteae chitinase

Table of Contents 1 Introduction 1 1.1 Structure and function of chitinases 1 1.2 Filarial chitinases are potential targets for interventions 4 1.3 A. viteae as a model to investigate parasitological parameters of filarial diseases 6 1.4 Aims and objectives of the study 7 2 Results 9 2.1 Characterisation of A. viteae chitinase genes 9 2.1.1 Determination of the gene structure of A. viteae chiti...